degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. Test Plates for absorbance and fluorescence intensity provide a complete traceable solution for validating optical performance of the SpectraMax M2 reader, automatically. Molecular Device SpectraMax M2 Plate Reader - Certified with Warranty. The reader temperature was set to 37 Celsius and most the the liquid in the wells of the plate had evaporated when I went to dispose of it. 4. We use Spectramax M5 microplate reader in our lab. used. Reader Documentation Version No. Choose from a three- (M3), four-. Five preferred detection modes. 9000-0756. SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 4 5039228D Chapter6:SpectraMax InjectorCartridge 79 InstalltheInjectorCartridge 79 UsingtheInjectorCartridge 86VAL014, right table) assays on the SpectraMax M5, M5e, or FlexStation 3 reader are shown below. The SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck® Pathlength Measurement Technology: the only temperature independent pathlength correction for microplates. For more sample throughput, the system canThe SpectraMax M5 plate reader which used to be in the Millennium Science Complex, W-124A is now located in the Proteomics and MS Core Facility, in 3 Althouse. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional. *This plate definition in SoftMax Pro Software is the same for black and white plates. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Molecular Devices SpectraMax M2e Multi-Mode Microplate Reader w/ SoftMax Pro. Choose the desired plate type, using the upper dropdown menu to choose plate format (96 orMolecular Devices Spectramax M5 Plate Reader is available from The Lab World Group. SpectraMax M2. Unplug the power cable from the back of the instrument. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader Specifications. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. 2. 25 pM (0. 1. 12. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. iD5. We show how the DuoLuc reporter assay and SpectraMax iD5 reader are used to detect nuclear factor-κB (NF-κB) activation in a mammalian cell model. The SoftMax® Touch Software is installed in. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). Description. There is currently no charge for using this plate reader. Human macrophages choreograph tissue development. SPECTRAmax PLUS Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS control panel are shown in boxed Helvetica type. SpectraMax M5eは、宇宙に打ち上げられた世界初のマイクロプレートリーダーです！ NanoRacksとモレキュラーデバイスとのパートナーシップ、そしてSpaceX Commercial Resupply Services MissonでSpectraMax M5eを打ち上げた取り組みの詳細については、こちらをクリックしてください。SpectraMax® i3xMulti-ModeMicroplateReader SpectraMax® i3Multi-ModeMicroplateReader FlexStation® 3Multi-ModeMicroplateReader SpectraMax® M5eMulti-ModeMicroplateReader SpectraMax®M5Multi-ModeMicroplateReader SpectraMax® M4Multi-ModeMicroplateReader SpectraMax® M3Multi. The computer controlling the plate reader runs Windows XP. Representative data from the SpectraMax i3x Multi-Mode Microplate Reader for both MycoAlert and MycoAlert PLUS assays are shown in Figure 3. 标准功. If the computer is connected to the Internet: Select Search Automatically for updated driver software. MICROPLATE READER: Used, in good condition, working microplate reader. 314 low lamp level warning. Plate chamber design of the SpectraMax category 1 microplate readers. SpectraMax M5e Multi-Mode Microplate Reader. FlexStation 3 reader Fluorescence Microplate Reader Comparison Chart . We therefore gathered salient. SpectraMax M5e Multi-Mode Microplate Reader. In the M2, M5 and Plus 384, the light. D 3 1. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. com. Our absorbance plate readers feature our PathCheck Sensor technology and. 00 USD. Data analysis Standard curves were plotted using the quadratic curve fit in SoftMax Pro Software, and the concentrations of cell lysates were calculated from the standard curves by the software. The protocol contains settings for reading the plate, as well as template groups that allow a user to easily set up analysis, including calculation. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. Luminescence was then measured using the microplate readers listed. l. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. 305 reference level saturation. A Costar® 24-well tissue culture plate with col1 = blanks and doubling cell concentration left to right starting at Col. The guide also covers the software features, the specifications, and the accessories of the systems. 1. The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. 0 out of 5 $ 15,999. 4. The microscopy module offers up to 60x magnification in fluorescence, brightfield, high contrast brightfield, color brightfield, and phase contrast to address many applications and workflows. There is currently no charge for using this plate reader. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based. spr SpectraMax iD3 SpectraTest ABS2 v1. The Gemini EM can sample a variety of formats, from 6- to 384-well microplates, in different modes, such as endpoint, spectral scan, kinetic, and well scan. Learn how you can utilize microplate readers to drive landmark discoveries. 000 OD. The substrate solution (100 μL/well) was subsequently employed for 5-min reaction and the absorbance at 370 nm was monitored on a SpectraMax M5 plate reader (Molecular Devices). The LMax plate reader has similar calculations and its RLUs are comparable to those of LMax II 384. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. Thermo Scientific Microplate Reader Verification Plates Cat# 24072800. Using an SpectraMax Microplate Reader for DNA absorbance test, there is one problem that the A260/A280 is always wrong, sometimes half plate wrong and sometime, several lanes. Spectramax i3x multi-mode microplate readers from molecular devices llc Spectramax m5 microplate reader (molecular devices) Molecular devices spectramax m5 microplate reader, sn: mv06308 100-240v Molecular devices spectramax i3x 使用される 販売のために 価格（税込） #9298540, 2017The optimized settings listed in Table 1 can be transferred to any plate reader of the same model, i. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. Supplier: Molecular Devices. The plate is set up for reading and images are acquired according to specified parameters. 8. Third this recommendation. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. SpectraMax M5 Multi-Mode Microplate Reader. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. 2. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. SpectraMax® M5 Microplate Reader Molecular Devices LLC. The reader has the most features and capabilities in one unit. Tailored to your different needs, these assay kits are configured and optimized for Molecular Devices SpectraMax® microplate readers, with preconfigured protocols provided in SoftMax® Pro Software for. Or, with the reader specifications noted from the Instrument Description section of your reader's manual, as well as those for your PC, you can calculate needs more closely. The Infinite and Spark multimode microplate readers are modular and upgradeable. The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. The calculated LOQ values were 0. Narrow your selection of used microplate readers using the filters above or select the one of the categories on the left to view multi-mode or. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. As a generous estimate, you might consider 300 W (at 120 Volts) per Spectramax reader, plus another 300 W for the Computer. Download PDF. Gemini EM Reader Top and Bottom OpticsThe SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Optimizing Fluorescence Polarization Assays Fluorescence polarization for SpectraMax M5 and M5 readers may only be read from the top of a microplate. Label-free detection. TNF-alpha quantification down to low picomolar levels on the SpectraMax Paradigm Platform. 2. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . 0200-0729. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. Abs). The system also gives users the flexibility to choose wavelengths between 200 nm and 1000 nm. Pleasanton, CA, USA. PAINS analysisA single-mode plate reader is a good starting point. youusehighprofile(6-well,12-well,24-well,or48-well)plates,youmustremovethe plateadapterfromtheplatedrawer. spr SpectraMax iD5 SpectraTest ABS2 v1. 8. To set up how to read the plate you can load an existing protocol from the protocol manager/protocol library or use the plate setup helper shown at the cursor below: 8. Dynamic Range. 000 to 4. spectramax multi-mode microplate Readers The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Extended linear dynamic range. FlexStation. Fluorescence Microplate Reader Comparison Chart. M5 and M5e Multi-Mode Microplate Readers User Guide SpectraMax M3, M4, M5. On LabX buyers can find a variety of SpectraMAX models for sale: 190, 384, 340 and the SpectraMAX M series. 58% for the detection module and 2. SpectraMax M5 is a multi-detection microplate reader with dual monochromators, a triple-mode cuvette port, and 6-384 well microplate reading capability. This reader's dual monochromators for. Read Modes Normal and Sweep Absorbance Measurement Range 0. Features. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. 2 Figure 3 shows the same assay plate read on the Analyst HT multi-mode reader. Instruments with a 96- or 384-well format will accommodate the most commonly used plates. Supersedes 1. M5 SpectraMax M5e Absorbance FluorescenceIntensity Luminescence Time-Resolved Fluorescence. Results. The SpectraMax® ABS Plus Microplate Reader can run . 3) At the bottom of the strip, click on "Show. At NanoRacks, the challenge for the engineers will be to modify the plate reader in order for the unit to operate comfortably in the zero-gravity environment of the space station. Thank you!. SpectraMax ® M5 Multi-Mode Microplate Reader Transcreener ® ADP. SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. Description The Onboard Microprocessor. The chamber. SpectraMax M5 Plate Reader SpectraMax M5 is a multi-detection microplate reader with dual monochromators, a triple-mode cuvette port, and 6-384 well microplate reading capability. Plate chamber design of the SpectraMax category 1 microplate readers. Certain functionalities in the table above requires specific configurations. PURPOSE. 3: The cuvette chamber. The standard curve was plotted using a log-log curve fit in the SoftMax® Pro Software (r2 = 1. [email protected] M3 Reader SpectraMax M4 Reader SpectraMax M5 Reader SpectraMax M5e Reader TRF,HTRF,FP HTRF, FP HTRF N/A Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well. Update Driver. IMAP assays are based on binding of. Both full length human PI4KIIIB and the truncated PI4KIIIB crystal construct with the c-term were run at 200 nM. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Also refer to this Multimode Reader Comparison Chart. Figure 1. Choose monochromator-based optics for flexibility, filter-based optics for sensitivity, or both…BioTek’s patented Hybrid Technology™ offers high-performance and applications versatility in a modular platform to expand as your laboratory’s. SpectraMax M5 reader SpectraMax M5e. Molecular Devices and can be performed only on an Molecular Devices plate reader. However, an upgradeable multi-mode reader which includes absorbance, fluorescence and luminescence can address all your future lab needs. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. Please contact Tatiana to reserve the plate reader time: tnl1@psu. The SpectraMax M5 plate reader which used to be in the Millennium Science Complex, W-124A is now located in the Proteomics and MS Core Facility, in 3 Althouse. Choose from a three- (M3), four- (M4), orProducts : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. The plate was vigorously mixed and incubated for 4 min at room temperature in the dark. The plate was incubated for 10 minutes at room temperature and read at 595 nm on the SpectraMax ABS Plus reader. Spectramax M5. 315 can't find zero order. The Analyst GT is equipped with 20 plate magazines in a bi-directional stacker. Robust, high-value microplate readers that can run samples based on pre-defined protocols and. GloMax® Discover is a ready-to-use multimode plate reader developed with Promega reagent chemistries to provide a simple means of detecting luminescence, fluorescence and absorbance. SpectraMax Glo Steady-Luc Reporter Assay Kit. 000 to 2. 1) Select the correct plate and the wells that you want to measure. Here is the problem: Using an SpectraMax Microplate Reader for DNA absorbance test, there is one problem that the A260/A280 is always wrong, sometimes half plate wrong and sometime, several lanes. Wokingham, Berkshire RG41 5TS United Kingdom. To update the driver. 12. 0 OD for our latest microplate readers, SpectraMax® iD3, iD5, ABS, and ABS Plus readers, and up to 2. , contact. A plate containing two columns each of 75 µL, 100 µL, 150 µL, 200 µL, 250 µL, or 300 µL of yellow buffer solution was read at 426 nm on a SpectraMax microplate reader. Software validation, IQ/OQ/PQ and FDA 21 CFR Part 11 compliance tools are also available. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. SpectraMax® M5/M5e Microplate Reader Have A Question? Contact Our Technical Support Team NA: 800-955-6288 Or INTL: 760-603-7200 Ext. NIST-traceable solution for validating the SpectraMax 340PC384 system. Qualify the absorbance performance of SpectraMax® iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, Plus 384, ABS, ABS Plus, and FlexStation 3 readers. Choose from a three- (M3), four- (M4), or Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. 8. 00800 665 32860. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Which SpectraMax M Series reader do you need? SpectraMax M Series Multi-Mode Microplate Readers 5 Ordering information VWR cat. Dual monochromators for assay flexibility With SpectraMax M Series readers, there is no need to utilize expensive filters to optimize detection levels and background. 0% ± 0. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Unlike filter-based plate readers, the dual monochromators in the SpectraMax iD5 Multi-Mode Microplate Reader and other SpectraMax readers allow the selection of any wavelength within the reader’s stated range. Maximum signal with lowest background. SpectraMax Gemini EM Microplate Reader A dual-monochromator spectrofluorometer system Dual monochromators With the Gemini EM Reader, users never. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. Our absorbance plate readers feature our PathCheck Sensor. begin reading microplates with seven mouse clicks. com 4. 000000 out of 5. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. Only Molecular Devices microplate plate readers offer the capability to . 40266 Email: [email protected] only) SpectraMax M-series Microplate readers. Fluorescein 0. Cytation 5 combines automated microscopy and conventional microplate detection in a configurable, upgradable platform. Choose from a three- (M3), four-. Cells in each image are identified by SoftMax Pro Software and cell-by-cell it is already turned on and the screen should look like this with “SpectraMax M5” showing as connected in the top left: 7. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. 000000 out of 5. 3752 in as per SBS dimensions. Considerations for readers include the measurement wavelength range, microwell plate capacity, throughput, detection methods, and a filter vs monochromator-based optical system. Gemini XPS & EM readers measure fluorescence on a variety of sample formats from 6- to 384-well. M5 and M5e Multi-Mode Microplate Readers User Guide SpectraMax M3, M4, M5. 8. SpectraMax M5 Plate Reader (Molecular Devices) If you have trouble accessing this page and need to request an alternate format, contact u@osu. 2 Figure 3 shows the same assay plate read on the Analyst HT multi-mode reader. It should work on the Spectramax L reader and also on the Spectramax M3, M4, and M5 plate readers (new firmware only) with Softmax Pro ® software version 5. HTRF-compatible SpectraMax Multi-Mode Readers. #3635 or Greiner cat. eu@moldev. ”The SpectraMax 190 reader can be integrated with Molecular . The assay window spanned four orders of magnitude. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 7 1. Equipped with scanning, shaking and kinetics modules. Microplate Readers Molecular Devices Spectramax M5 Microplate Reader. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. com. The drawing shows a standard microplate of 15 mm height covered with a lid. Catalog Number: M5. 8. com 10. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. Shop now. The SpectraMax® iD3 Multi-Mode Microplate Reader and the SpectraMax® iD5 Multi-Mode Microplate Reader from Molecular Devices® have a touch screen interface that provides integrated instrument control, data display, and the ability to export results over your network for statistical data analysis. This process should take about 3 - 5 minutes. 0112-0115 F . TNF standard curve. 000). SpectraMax Injector Cartridge with SmartInject™ Technology. Dynamic Range. 0 OD for earlier models. Description 1. SpectraMax Paradigm Multi-Mode Safety Guide SoftMax® Pro Software is the most published microplate reader control and data analysis software in the industry. Align the two slots on the bottle holder with the two pegs on the rail. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. 6 for further experiment, so I'm confused which i would choose for OD value determination. " SpectraMax plate reader is very easy to use, with detection of absorbance, fluorescence, luminescence, etc. 303 unable to cal dark current. Description 1. 7°C and the bottom to 36. measure the depth (optical pathlength) of samples with no temperature. Monitor reader performance on demand to ensure data quality is not compromised between PM/OQ. Category 2 and 3 microplate readers use gradient heating as well. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. Fluorescence Microplate Reader Comparison Chart. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection Platform. The system also gives users the flexibility to choose wavelengths between 200 nm and 1000 nm. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. With only a few product lines that we provide service for, our engineers have unrivaled expertise in the SpectraMax® microplate readers. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. 000 to 3. SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. Perkin Elmer 1420-2560 Victor X Series Plate Reader Sipper. The instrument settings were identical to those used in Example 1 above. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. 40266 Email: drugdiscoverytech@lifetech. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode…. Figure 1. Fill a clean plate with water. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. . i3. Specifications Plate mixing modes Selectable: off, once prior to any reading, and once prior to and between kinetic readings Plate mixing duration Selectable: 0 to 999 seconds (three-second default. Click the OK button. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry-leading SoftMax Pro. The following document is. . SpectraMax L reader, SpectraMax M5/M5e reader What is the StakMax timing with SpectraMax Plate Readers? If using the StakMax and a SpectraMax reader together, the time specifications for the movement of the StakMax are as follows:. 010 OD 384-Well Plate,. The SpectraMax M5 plate reader which used to be in the Millennium Science Complex, W-124A is now located in the Proteomics and MS Core Facility, in 3 Althouse. In other words, I carry out equilibrium folding/unfolding titrations of proteins and monitor the process using. Rating: 5. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. A preconfigured protocol with optimized reader settings enabled reading of a 384-well plate in just two minutes. 2 Software (multiple versions available) Windows 7 PC 60 day warranty All necessary communication electrical cables The upgradeable SpectraMax M5 Multi-Mode Microplate Reader comes with the standard five modes of detection with the upgrade option to add HTRF at a later time. The SpectraMax M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck Pathlength Measurement Technology: the only temperature independent pathlength correction for microplates. The Molecular Devices Spectramax M5 Multi-Mode Plate Reader brings you the power and precision of a dedicated single mode plate reader, but with the space saving flexibility of 5 detection modes in one unit. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. Wide range of applications The VersaMax reader covers a wide range of applications:Molecular Devices. 11. Figure 1. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. 2. The SpectraMax ® i3x. Prepare your i3/i3x instrument for shipment. 5 pM (0. In these readers, RLUs are proportional to counts/sec which doesn't change with integration time assuming the. e. Description 1. These lab workhorses are used in drug discovery. 1. Microplate Reader Shelf. Instructions on the proper use of the SpectraMax M5 Plate Reader. SpectraMax® Microplate Readers and SoftMax® Pro Software. It does not work as expected. The plate reader can measure multiple readouts, including UV. SpectraMax® M2 Multi-Mode Microplate Reader MLDVM2 SpectraMax® M2e Multi-Mode Microplate Reader (top and bottom reading) MLDVM2E SpectraMax® M3 Multi-Mode Microplate Reader MLDVM3. Plate Shaking Linear, Orbital Slow, and Orbital Fast. Wide range of applications. For the BioVision cytotoxicity kit, 100 µL of culture medium was removed from each treated well and transferred to a solid white plate. free tracer decreases, resulting in an. The SpectraMax M5 plate reader which used to be in the Millennium Science Complex, W-124A is now located in the Proteomics and MS Core Facility, in 3 Althouse. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). 40266 Email: drugdiscoverytech@lifetech. 2. After the plate is read, data will appear in the plate section: 10. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. Prepare your iD series instrument for shipment. The plastic from a microplate will affect the light polarization, precluding bottom reads and reading a covered plate. Cells in each. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. Lower end of DNA standard curve. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Similar settings will work for other SpectraMax readers with fluorescence detection. 1. The upgradeable SpectraMax M5 Multi-Mode Microplate Reader comes with the standard five modes of detection with the upgrade option to add HTRF at a later time. D 1 1. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. com 4. The SpectraMax M5 uses standard microplates to read endpoint, kinetic, spectrum, multi-wavelength and. Products : SpectraMax M3 reader, SpectraMax M4 reader, SpectraTest Validation Plates FL1, Gemini EM reader, Gemini XPS reader, SpectraMax M5/M5e reader My SpectraTest FL one plate is failing for LLD for the wet test. Shake. com 4. The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. Here the delta mP was 336, and Z’ factor was 0. For wavelengths below 220 nm, only quartz plates are to be used. ". Bottom-reading is usually recommended for cell-based assay with adherent cells. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port. MichiganSpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. Plates were measured at 450 nm with 650 nm as a reference (Molecular Devices, SpectraMax M). Set up Identify Acquire Analyze Imaging with the SpectraMax MiniMax Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3 System. Type Clear: Add to Cart. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader. This Molecular Devices SpectraMax iD5 Microplate Reader is made in Oct. 000 OD ± 1. MD Spectramax iD5 Plate Reader. SpectraMax Glo Steady-Luc Reporter Assay Kit workflow. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. Pathcheck Pathlength Measurement Technology. ) Open SoftMax Pro, set Preference to SpectraMax L, and try the different available COM ports to find the one for the SpectraMax L. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v2. SpectraMax i3x Multi-Mode Microplate Re. Before you do a bottom read, or use high profile (6-well, 12-well, 24-well, or 48-well) plates, you must remove the plate adapter from the plate drawer. Wait 20 seconds. It is important to determine the excitation and emission wavelengths that provide the best dynamic range for the assay, since these may differ somewhat from those used with filter-based plate readers. Most papers they cultured bacteria to OD600>0. The plate reader. The drawing shows a standard microplate of 15 mm height covered with a lid. (128) 100%. it is already turned on and the screen should look like this with “SpectraMax M5” showing as connected in the top left: 7. Table of Contents. 40266The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. 2. 04 to 3. SpectraMax iD5 Multi-Mode Microplate Reader, a hybrid monochromator- and filter-based, five-mode plate reader with automatic filter identification and western blot capability. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual.